Effect of cleavage of the p220 subunit of eukaryotic translation initiation factor eIF-4F on protein synthesis in vitro.

0.6 30 % 96 % 0.1 3 % 65 % 0.6 28% 67 % The Foot and Mouth Disease Virus (an aphthovirus within the picomavirus family), encodes a protease called L which is believed to cause the proteolysis of the p220 subunit of the initiation factor eIF-4F[ 11. The role of p220 in the initiation of translation still remains obscure but previous studies on this polypeptide showed that it is necessary for efficient translation[2]. Indeed, the cleavage of p220 by some picomavirus encoded proteases results in the shut-off of host protein synthesis, while picomavirus translation is unaffected by the lack of intact